Journal:
Article Title: Disruption of SEMA4D ameliorates platelet hypersensitivity in dyslipidemia and confers protection against the development of atherosclerosis
doi: 10.1161/ATVBAHA.109.185405
Figure Lengend Snippet: (A) Aortas after 6 months on the high fat diet showing lipid-rich deposits. (B,C,D) Analysis of lesion size. (E) Sections from the aortic root. Markers: CD4 and CD8, T-cells; CD22, B-cells; CD11b, monocytes, macrophage and neutrophils; smooth muscle actin (αSMA), smooth muscle cells and fibrotic caps. No differences were observed.
Article Snippet: Acetone fixed and peroxidase quenched sections were incubated with biotinylated rat anti-mouse CD4 and rat anti-mouse CD8 (made in-house), rat anti-mouse CD22 (Southern Biotech, Birmingham, AL) and rat anti-mouse CD11b (BD Biosciences, Pasadena, CA) and FITC-conjugated mouse anti-α-smooth muscle actin (SMA) clone 14A (Sigma, St. Louis, MO).
Techniques: